EffectofPorousActivatedCharcoalReinforcementonMechanicalandIn-VitroBiologicalPropertiesofPolyvinylAlcoholCompositeScaffolds

摘要： Thepresentworkfocusedondevelopinganinnovativecompositematerialbyreinforcingpolymermatrixwithhighlyporousactivatedcharcoal.Polyvinylalcohol-activatedcharcoal(PVA-AC)compositescaffoldsweredevelopedbyvaryingtheACconcentrations(0,0.5,1,1.5,2and2.5wt%)inPVAmatrixbyfreezedryingmethod.Thedevelopedscaffoldswerecharacterizedfortheirphysicochemical,mechanicalandin-vitrobiologicalproperties.Inaddition,theeffectofAContheattachment,proliferationanddifferentiationofosteoblastMG63cellswasevaluatedbyscanningelectronmicroscopy(SEM),3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide(MTT)assay,alkalinephosphatase(ALP)activityassayandalizarinredstain-based(ARS)assay.AllthePVA-ACcompositescaffoldsexhibitedgoodbioactivity,hemocompatibilityandproteinadsorptionproperties.ThescaffoldswithhighACconcentration(2.5wt%)showedcontrolleddrugreleasekineticsthataresuitableforlongtermhealing.ThemechanicalpropertiesofallthePVA-ACcompositescaffoldswereimprovedwhencomparedtothepurePVAscaffold.Thehighporosity,swellingdegreeandhydrophilicityofPVA-ACcompositescaffoldsfacilitatedcellattachmentandproliferation.ThisisduetoporousACpresentinthesamplethatsupportedtheosteoblastdifferentiationandformedmineralizednoduleswithouttheadditionofanyextraagents.Fromtheabovestudies,itcanbeconcludedthatPVA-ACcompositescaffoldsarepromisingbiomaterialsforbonetissueengineeringapplications. （共10頁(yè)）